Ruxshona Izzatbekovna Toxirova
Moderator 
DETECTING THE PRESENCE OF THE ENZYME URIASE IN BEANS
Abstract:
This article examines the botanical characteristics, chemical composition, and medicinal
properties of the Phaseolus plant. Special emphasis is placed on its use in traditional
medicine, its richness in vitamins, and the discovery of the presence of the enzyme urase.
Keywords: Phaseolus, urase, urea, thiourea, phenolphthalein.
Introduction
Phaseolus (Beans) is an annual vegetable belonging to the legume family. Originating from
Central and South America, it is widely cultivated in India, Brazil, China, and Uzbekistan.
Among vegetables, beans are notable for their rich content of various vitamins,
macronutrients, and micronutrients. A single can of canned beans provides essential
energy for the human body, containing 0.7 g of fats, 14.5 g of proteins, and 4 0 g of
carbohydrates per 100 grams. It also contains both water-soluble and fat-soluble vitamins
such as B2, B12, C, E, and K, and is a protein-rich plant.
Health Benefits of Beans
Different varieties of beans—mainly white, red, and green—are consumed, each with
unique benefits. Beans help strengthen blood vessels, aid in weight management, reduce
cholesterol, and regulate blood sugar. Additionally, they promote digestion and contain
potassium for heart health, magnesium for nerve support, and zinc for immune
enhancement. Vitamin C helps rejuvenate cell walls, while magnesium contributes to
various bodily reactions, including metabolism. Beans are a good meat substitute and
provide livestock feed. During pregnancy, consuming beans supports the daily need for
macro and micronutrients, positively affecting fetal development.
Practical Application
To detect the enzyme urase in beans, we began by grinding white and red beans into a fine
powder.
White Beans
In the lab, we took two clean test tubes. We added six drops of urea to the first tube and six
drops of thiourea to the second. We then added two drops of phenolphthalein to each tube
and added the powdered white bean (containing urase) to both. After half an hour at room
temperature, the solution in the first tube turned pink. This is because the urase enzyme in
the beans broke down the urea, releasing NH3 gas, which formed an alkaline solution that
turned the phenolphthalein pink.
Red Beans
In the second experiment, we repeated the steps with powdered red beans. Again, the
solution in the first test tube turned pink, indicating that the urase in red beans also broke
down the urea, releasing NH3 gas and creating an alkaline environment that turned the
solution pink.
Conclusion
In conclusion, equal amounts of the enzyme urase were found in both white and red beans.
Ruxshona Izzatbekovna Toxirova was born on July 25, 2004, in the Oltinkoʻl district of Andijan region. She is Uzbek by nationality. From 2011 to 2022, she studied at the 48th general secondary school in the Oltinkoʻl district. Currently, she is a second-year student at the Faculty of Pediatrics at Andijan State Medical Institute. She graduated from school with excellent grades and achleved numerous successes, actively participating in subject Olympiads. She is the coordinator of the Girls' Club. She participated in the conference "INNOVATIVE APPROACH TO CURRENT ISSUES IN MEDICINE" held on March 29, 2024. She is also the author of many articles.
The article was published without interference!
